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Initially PCR was executed by using exon 4a-specific ahead primer (4a-F, 5'ATTCCATTGCCTTTCCACAG-3') and 3' RACE outer reverse primer (5'-GCGAGCACAGA ATTAATACGACT-3'). Nested PCR was performed through the use of the primary PCR product or service like a template together with the nested Ex4a(+)WT1 cDNA-specific ahead primer (4a-F2, 5'-GCAAAGCTCT CTGTG ACATTA-3') as well as nested adaptor interior p
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In 1954, Joan Miro received a prize for printmaking in Venice Biennale. He also won the Guggenheim International Award in 1958 and obtained the Gold Medal for Wonderful Arts from King Juan Carlos of Spain in 1980. The artist attained his doctorate 'honoris causa' from the University of Barcelona in the yr 1979. In 1976, his museum, the Fundació Joan Miró, Centre d'Estudis d'Art Contemp

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