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Capan-1 is a especially intriguing cell line as it is both BRCA2 deficient, like several familial breast cancers , nevertheless derived from a pancreatic adenocarcinoma. Though two BRCA2-deficient tumours have previously been assessed by massively parallel DNA sequencing, this was only at low depth, sufficient for studies of chromosomal rearrangements, but not SNPs and indels .
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N InsDel (rs3834129) and CASP8 Asp302His (rs1045485), were repeatedly associated with reduced breast cancer susceptibility. Contrarily, the presence of the -652 6N Del or the CASP8 302His variant was reported to be an unfavorable prognostic factor in colorectal cancer or neuroblastoma. However, prognostic relevance of these genetic variants for breast cancer is completely unknown and is therefore
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